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XYbio Recombinant Human Interleukin-6(IL-6)重组人白细胞介素-6

货号:X13250

品牌:XYbio

规格:5ug 100ug 500ug

产品介绍

订购信息

货号 描述 规格 单价/元
X13250-5ug Recombinant Human Interleukin-6(IL-6)重组人白细胞介素-6 5ug 817
X13250-100ug Recombinant Human Interleukin-6(IL-6)重组人白细胞介素-6 100ug 7478
X13250-500ug Recombinant Human Interleukin-6(IL-6)重组人白细胞介素-6 500ug 16237

    Synonyms
    BSF-2, CDF, Hybridoma growth factor, IFN-beta-2.
    Accession
    P05231
    GeneID
    3569
    Source
    Escherichia coli.
    Molecular Weight
    Approximately 20.8 kDa, a single non-glycosylated polypeptide chain containing 183 amino acids.
    AA Sequence
    VPPGEDSKDV AAPHRQPLTS SERIDKQIRY ILDGISALRK ETCNKSNMCE SSKEALAENN LNLPKMAEKD GCFQSGFNEE TCLVKIITGL LEFEVYLEYL QNRFESSEEQ ARAVQMSTKV LIQFLQKKAK NLDAITTPDP TTNASLLTKL QAQNQWLQDM TTHLILRSFK EFLQSSLRAL RQM
    Purity
    > 96 % by SDS-PAGE and HPLC analyses.
    Biological Activity
    Assay #1: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg.
    Assay #2: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine T1165 cells is less than 0.8 ng/ml, corresponding to a specific activity of > 1.25 × 106 IU/mg.
    Physical Appearance
    Sterile Filtered White lyophilized (freeze-dried) powder.
    Formulation
    Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
    Endotoxin
    Less than 1.0 EU/µg of rHuIL-6 as determined by LAL method.
    Reconstitution
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
    Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    - 12 months from date of receipt, -20 to -70 °C as supplied.
    - 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
    Usage
    This material is offered by xybio  for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
    SDS-PAGE
    Reference
    1. Ferguson-Smith AC, Chen YF, Newman MS, et al. 1988. Genomics. 2:203-8.
    2. van der Poll T, Keogh CV, Guirao X, et al. 1997. J Infect Dis. 176:439-44.
    3. Ming JE, Cernetti C, Steinman RM, et al. 1989. J Mol Cell Immunol. 4:203-11; discussion 211-2.
    4. Bastard JP, Jardel C, Delattre J, et al. 1999. Circulation. 99:2221-2.
    5. Heinrich PC, Behrmann I, Muller-Newen G, et al. 1998. Biochem J. 334 ( Pt 2):297-314.
    Background
    Interleukin-6 (IL-6) is an interleukin that in humans is encoded by the IL-6 gene and acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response. Furthermore, It plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It also induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. The human IL-6 is a single non-glycosylated polypeptide chain containing 183 amino acids and it signals through a cell-surface type I cytokine receptor complex consisting of the ligand-binding IL-6Rα chain (CD126), and the signal- transducing component gp130 (also called CD130). The human IL-6 shares about 40% a.a. sequence identity with mouse and rat IL-6 and it is equally active on mouse and rat cells.

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